Anna Osadcenko was born in Almaty in Kazakhstan. She received her ballet training at the Academie A. B. Selesnew as well as at the John Cranko Schule in Stuttgart, from which she graduated in July 2001. After being an apprentice with the Stuttgart Ballet, Anna Osadcenko became a Corps de ballet member in 2002/03. In 2008/09 she was promoted to Principal Dancer.

Her repertoire includes a wide range of leading and solo roles such as Odette/Odile in Swan Lake (John Cranko), Tatiana in Onegin (John Cranko), Juliet in Romeo and Juliet (John Cranko), Katharina in The Taming of the Shrew (John Cranko), the Sylph in La Sylphide (Peter Schaufuss after August Bournonville), Princess Aurora in The Sleeping Beauty (Marcia Haydée after Petipa), Queen of the Dryades in Don Quixote (Maximiliano Guerra after Petipa) and Olympia in The Lady of the Camellias (John Neumeier). In addition, Anna Osadcenko has performed in ballets by renowned choreographers such as George Balanchine, William Forsythe, Jiří Kylián, Sir Kenneth MacMillan, Glen Tetley and Hans van Manen. Several choreographers including Sidi Larbi Cherkaoui, Christian Spuck, Douglas Lee and Jorma Elo have created roles for her.